Ammonia Affects Astroglial Proliferation in Culture.

Ammonia Affects Astroglial Proliferation in Culture.

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Primary cultures of rat astroglial cells were exposed to 1, 3 and 5 mM NH4Cl for up to 10 days.Dose- and time-dependent reductions in cell numbers were seen, plus an increase in the proportion of cells in the S phase.The DNA content was reduced in the treated cells, and BrdU incorporation diminished.

However, neither HYDRATE CONDITIONER ammonia nor ammonia plus glutamine had Refrigerators:Merchandising Glass Door Refrigerators any effect on DNA polymerase activity.iTRAQ analysis showed that exposure to ammonia induced a significant reduction in histone and heterochromatin protein 1 expression.A reduction in cell viability was also noted.

The ammonia-induced reduction of proliferative activity in these cultured astroglial cells seems to be due to a delay in the completion of the S phase provoked by the inhibition of chromatin protein synthesis.